Western blot analysis of CD95L expression in a Th1 and in a Th2 clone. Clones 103 (Th1) and 3/39 (Th2) were sensitized to gp120-apoptosis or stimulated with PMA/ionophore; after 1 hour, samples were collected and processed for Western blot analysis. Lane 1, untreated; lane 2, gp120/anti-gp120/antiTCR; lane 3, PMA/ionophore.