Both Th1 and Th2 clones are susceptible to anti-CD95 MoAb-induced apoptosis. Two Th1 (103 and 1/36) and two Th2 clones (2/46 and 3/39) were cultured in medium only or with the indicated doses of anti-CD95 agonistic MoAb CH-11. Cells were collected after 4 hours and stained by TUNEL. The percentage of CH-11 apoptosis was calculated by subtracting background apoptosis in medium alone (15%, 21%, 5%, and 10% in clones 103, 1/36, 2/46, and 3/39, respectively), to that obtained in the presence of the indicated doses of MoAb.