Western blotting analysis of hematopoietic cell populations with the anti-runt box antiserum. (A) Lysates from total bone marrow cells (Tot) or from bone marrow subpopulations enriched for expression of CD45R, Gr-1 (GR), and TER-119 (TER). pre, preimmune serum; anti-rbox, anti-runt box antiserum. (B) Lysates from 12.5- and 16.5-dpc liver cells. (C) Lysates from peritoneal macrophages (Mφ ), thymus, and spleen cells. The total bone marrow lysate (BM) is shown for reference. In all panels, each lane corresponds to 10⁷ cells. Specifically detected polypeptides are indicated by the open arrowheads on the left, with the estimated molecular weight given. Asterisks mark nonspecific bands (see text). The positions of the molecular weight markers are indicated on the right (solid arrows).