Sickle RBC adhesion to slides sequentially coated with TSP and vWF. TSP and vWF were sequentially coated onto glass microscope slides (as depicted). A washed suspension of RBC (1% hematocrit in serum-free medium) was perfused into the flow chamber for 10 minutes, followed by a 10-minute rinse with cell-free medium at a shear stress of 1 dyne/cm². The number of adherent RBC remaining at the end of the rinse period was determined using video microscopy. vWF-induced inhibition of sickle RBC binding to TSP was observed irrespective of the order of protein application to the slide.