Effect of hydrocortisone on plasminogen mRNA expression in primary murine hepatocytes. Primary murine hepatocytes were grown in serum-free EMEM supplemented with 0.1% BSA for 24 hours, followed by culture for 24 hours in the presence of either 10 μmol/L hydrocortisone, 500 U/mL rhIL-6 or hydrocortisone + rhIL-6 at the foregoing concentrations in serum free EMEM containing 0.1% BSA. Total RNA was isolated and Northern blot analysis was performed as in Fig 1. Migration of RNA molecular size standards is shown on the left side of the panel.