Fig. 2.
![Fig. 2. E64d blocks anti-CD3–induced upregulation of Fas ligand protein. 2B4.11 cells were activated with the indicated concentrations of plastic-coated 2C11 in the presence or absence of 100 μmol/L E64d for 2 hours. [3H]thymidine-labeled L1210 cells or L1210-Fas cells were then added to the cultures. The parentheses in the legend indicate the time of addition of E64d: (2B4.11 + E64d), E64d added at time 0; (E64d + L1210-Fas), E64d added with the L1210-Fas cells, 2 hours after initiation of the culture. After another 6 hours of incubation, the cells were harvested and the percentage of DNA fragmentation determined. Similar results were obtained in three independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/2/10.1182_blood.v89.2.550/4/bl_0001f2.jpeg?Expires=1722687809&Signature=jmdIpEbRo~6liMLNKe4iAhzgtpDaP6Zj6ygGZAirlxK~bBhEH6iOkq1beE3riQPGFH2E7q9v2jHDO3bEmpb-wYVgIUV~gEaJmfbJDr26M48jjYQZvB6Zq6lIjD3cF51vCcYj8GowUOpD63-DyuHpiXP08pX9RTSfuwabP4bXxJlVTkQxf~ZgR-Q3ym~5lGkLjoAIc0OPHSmLvZpYRGcn6-OerGxyqipMEkdSJ5oZJVtZfoQGL7xvPL57LWHGYRC16jsAfg0yYsLHYJXR3acF~dLoj04y6mE0u8BI-xjwD3OKxpSB4kLlZgTO6GB8rQ9iW-c1pURmUfrGlGlh5q4P~A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
E64d blocks anti-CD3–induced upregulation of Fas ligand protein. 2B4.11 cells were activated with the indicated concentrations of plastic-coated 2C11 in the presence or absence of 100 μmol/L E64d for 2 hours. [3H]thymidine-labeled L1210 cells or L1210-Fas cells were then added to the cultures. The parentheses in the legend indicate the time of addition of E64d: (2B4.11 + E64d), E64d added at time 0; (E64d + L1210-Fas), E64d added with the L1210-Fas cells, 2 hours after initiation of the culture. After another 6 hours of incubation, the cells were harvested and the percentage of DNA fragmentation determined. Similar results were obtained in three independent experiments.
