RT-PCR analysis of expression of the adult α- and β-globin mRNA in zebrafish embryonic blood at 24 hours, 2 days, 4 days, and 10 days; in a whole adult zebrafish; in a zebrafish adult spleen; and in zebrafish adult blood. EF1-α is used as a control for RNA levels. A reaction with water (lane 4) is used as a negative control.