In vitro differentiation of myeloid cells after transduction. Mock- or vector-transduced CD34⁺ cells were differentiated for 10 days and analyzed for the myeloid marker CD14 and lyt2 expression (A). Kinetic analysis indicated stable lyt2 expression in the myeloid lineage cells in CD34⁺ cells transduced with either LMiLy or LΔMiLy (B). The presence of RevM10 gene product (LMiLY or LΔMiLy) did not affect the efficiency of differentiation.