(A) Quantitative RT-PCR of RFC1 expression in WT MOLT-3 cells, MOLT-3/MTX₂₀₀ cells and MOLT-3/MTX_10,000 cells. G3PDH expression is used as a control. (B) Flow cytometric analysis of WT MOLT-3 (top) and MOLT-3/MTX_10,000 cells (bottom) with anti-RFCl polyclonal antiserum and control preimmune serum. The X-axis is fluorescence intensity and the Y-axis is cell number.