Fig. 7.
![Fig. 7. Scatchard analysis of transferrin binding to K562 cells. Cells were preincubated for 24 hours with 50 U/mL human recombinant Epo (Epo), 100 μmol/L desferrioxamine (Dfo), or no addition (C) and were then incubated for 30 minutes on ice with 125I-transferrin. Nonspecific binding was determined by incubating with a 200-fold excess of unlabeled transferrin. The number of bindings sites per cell after correcting for nonspecific binding were, for control, n = 0.45 × 105 (dissociation constant [kd] = 41 nmol/L); for Epo-treated cells, n = 1.11 × 105 (kd = 43 nmol/L); and for desferrioxamine-treated cells, n = 2.92 × 105 (kd = 43 nmol/L). The results of one of three similar experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/2/10.1182_blood.v89.2.680/4/bl_0026f7.jpeg?Expires=1724253601&Signature=iAhEKN6krLhDpJzeAKnhC0d2bBGJb3vpf0WP82p7Yl-tNi0oLIB3p7AJV-DwgSmr9A0YpI8plTRZ1QgFOjv77KErkA-imOY1VxYHtSUIOlDMX7S0vDGjSawmWddityoBQlNwtBmLnp1TO5XMmMarhkPz7UQS0ruaDbvxBtSp58ytzWViQ5p~jBH405lmPTdN6FAkrNSB1F44NHFliI73gphn5EMwyIZ-5xnvLewSpBzjrgl~b4EYtjAMcYNFYGoR77dlvmEpAtHlci1ohlZDnNMqiakIMxXOTgP9nVEG2nFnZo6i8wYYPwihPeeDrr9DRLb72n354XZLvyPpS-mmqQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Scatchard analysis of transferrin binding to K562 cells. Cells were preincubated for 24 hours with 50 U/mL human recombinant Epo (Epo), 100 μmol/L desferrioxamine (Dfo), or no addition (C) and were then incubated for 30 minutes on ice with 125I-transferrin. Nonspecific binding was determined by incubating with a 200-fold excess of unlabeled transferrin. The number of bindings sites per cell after correcting for nonspecific binding were, for control, n = 0.45 × 105 (dissociation constant [kd] = 41 nmol/L); for Epo-treated cells, n = 1.11 × 105 (kd = 43 nmol/L); and for desferrioxamine-treated cells, n = 2.92 × 105 (kd = 43 nmol/L). The results of one of three similar experiments are shown.
