Fig. 1.
![Fig. 1. Activation of IRE-binding activity of IRP by Epo in K562 cells. K562 cells were treated for 24 hours with 50 μmol/L Fe(NO3 )3 (I), 100 μmol/L desferrioxamine (D), 50 U/mL human recombinant Epo (Epo), or 1.5% DMSO or remained untreated (control [C]). Fifteen micrograms of detergent cell extracts was assayed for IRE-binding activity of IRP by means of gel shift assay in the absence (upper panel) or presence of 2% 2-mercaptoethanol. After fixation, gels were exposed for 6 to 48 hours to x-ray films. The IRE/IRP complex and the nonbound IRE-probe (free probe) are shown in the upper panel, whereas in the lower panel only RNA-protein complexes are pictured. The results of one of seven similar experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/89/2/10.1182_blood.v89.2.680/4/bl_0026f1.jpeg?Expires=1724253748&Signature=vOWg81odKO2BTbCqYgaQvXsOUaULwzCxppOcOlwoX67i0OGiEwibATACXc-HdJ~Ua9wz3F-~k-EhYERoHMYxRXKCE8X-ZajRwIvYtDPhteuwxkaAgjZl072s8Khvl222mq9QigjDTD6SOcL73GhQLMlGumsm1cHr1vbdGb~xKWGyd6zuqCOZZ3x3yxv2fiPj8OhAw7zifOQgs1rncb~guY~aN2kMGX8eg3E06pjqj7kMd9s91PU3s74VYYN4rt7TsRulAGFh4GcXBVhFMwjKHK3zpnzqoK5A1bwjFGnHwZB90ga7CGqQm4A67Md4KwaMUF~i03kmBIQ047Nfc1Gciw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of IRE-binding activity of IRP by Epo in K562 cells. K562 cells were treated for 24 hours with 50 μmol/L Fe(NO3 )3 (I), 100 μmol/L desferrioxamine (D), 50 U/mL human recombinant Epo (Epo), or 1.5% DMSO or remained untreated (control [C]). Fifteen micrograms of detergent cell extracts was assayed for IRE-binding activity of IRP by means of gel shift assay in the absence (upper panel) or presence of 2% 2-mercaptoethanol. After fixation, gels were exposed for 6 to 48 hours to x-ray films. The IRE/IRP complex and the nonbound IRE-probe (free probe) are shown in the upper panel, whereas in the lower panel only RNA-protein complexes are pictured. The results of one of seven similar experiments are shown.
