Cell cycle analysis of purified c-kit⁺ Thy-1.1^lo Lin^−/lo Sca-1⁺ cells from splenectomized mice after G-CSF mobilization. Mice were splenectomized and, after 7 weeks, infused continuously for 7 days with G-CSF (250 μg/kg/d). c-kit⁺ Thy-1.1^lo Lin^−/lo Sca-1⁺ cells were purified and the cells were stained with Hoechst 33342 for analysis of DNA content. The FACS plots show Hoechst 33342 staining versus forward scatter of c-kit⁺ Thy-1.1^lo Lin^−/lo Sca-1⁺ cells isolated from the (a) BM or (b) MPB of G-CSF–treated mice. The percentage of cells in G0/G1 or S/G2/M is indicated on the left or right side of each FACS plot, respectively.