Cell cycle analysis of purified mouse c-kit⁺ Thy-1.1^lo Lin^−/lo Sca-1⁺ cells after mobilization with G-CSF. Mice were infused for 7 days with of G-CSF (250 μg/kg/d). c-kit⁺ Thy-1.1^lo Lin^−/lo Sca-1⁺ cells were purified and stained with Hoechst 33342 for analysis of DNA content. The data from two replicatel experiments are shown. The FACS plots display Hoechst 33342 staining versus forward scatter of c-kit⁺ Thy-1.1^lo Lin^−/lo Sca-1⁺ cells isolated from (a and e) BM of PBS/BSA mock-treated mice or from G-CSF–treated (b and f ) BM, (c and g) spleen, and (d and h) MPB. The percentage of cells in G0/G1 or S/G2/M is indicated on left or right side of each FACS plot, respectively.