Influence of inhibition of Notch signaling with DAPT on development in hybrid human-mouse FTOC seeded with human CD4⁺ISP thymic progenitor cells. Murine fetal thymic lobes were seeded with human CD4⁺ISP thymic progenitor cells cultured for 10 days in the absence or presence of 5 μM DAPT. Representative dot plots of the different subpopulations of 3 independent experiments are shown.