Figure 4.
Figure 4. Inhibition of Notch signaling affects VDJβ but not DJβ rearrangements. Southern blots are shown of the products of PCR performed on cell lysates of control or DAPT-treated FTOCs, which were originally seeded with CD34+CD1- human thymic progenitor cells and were cultured for 20 days. DJβ (left) and VDJβ (right) rearrangements are shown for undiluted and 1:3 dilutions of the PCR products. Lanes 1 to 3 represent samples from untreated FTOC or FTOC in the presence of 2 or 5 μM DAPT, respectively.

Inhibition of Notch signaling affects VDJβ but not DJβ rearrangements. Southern blots are shown of the products of PCR performed on cell lysates of control or DAPT-treated FTOCs, which were originally seeded with CD34+CD1- human thymic progenitor cells and were cultured for 20 days. DJβ (left) and VDJβ (right) rearrangements are shown for undiluted and 1:3 dilutions of the PCR products. Lanes 1 to 3 represent samples from untreated FTOC or FTOC in the presence of 2 or 5 μM DAPT, respectively.

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