PAL-E antigen is not vimentin. (A) PV-1–transfected CHO cells and their mock-transfected controls were surface-stained with the antibodies indicated in the figure and analyzed by flow cytometry. The x-axis represents the fluorescence intensity, and y-axis represents the relative number of cells. (B) HMEC lysate was precleared with the indicated antibodies; thereafter, the samples were run on SDS-PAGE and blotted to nitrocellulose membranes. Detection was performed with antivimentin antibody (V9) or with a negative control antibody (3G6). Molecular weight markers are indicated on the left. The arrow points to the vimentin band.