Mechanistic analysis of the selectin binding properties of WT, T-bet^–/–, DO11.10, and DO11.10 × T-bet^–/– primary CD4⁺ T cells under conditions of shear flow. (A-B) Interaction of primary CD4⁺ T cells of different genotypes with immobilized P-selectin (left columns) and E-selectin (right columns) under conditions of laminar shear stress as indicated. (A) Wild-type (WT, □) and T-bet^–/– (▪) CD4⁺ Th1 cells activated by polyclonal stimulation with CD3 and CD28 antibodies. (B) CD4⁺ T cells generated from DO11.10 and DO11.10 × T-bet^–/– TCR-Tg animals and activated in an antigen-specific manner. (C-D) Interaction of WT (□) and T-bet^–/– (▪) Th1 and Th2 cells, activated with plate-bound CD3 and CD28 antibodies, with (C) immobilized P-selectin and (D) E-selectin under conditions of laminar shear stress as indicated. All data are expressed as mean ± SEM.