In vivo thrombus formation and bleeding times in wt, SLAM–/–, and SAP–/– mice. (A) Intravital microscopy of thrombus formation after FeCl3 injury of the mesenteric artery in young C57Bl/6 wt (black), SLAM–/– (blue), and SAP–/– (red) mice was determined as described.13 The deposition of rhodamine 6G–labeled platelets at site of vessel wall injury (thrombotic profile) was determined by intravital microscopy of the mesenteric artery of 3-week-old mice; one vessel was studied per mouse. The increase in fluorescence intensity relates in a linear fashion to the number of platelets.%13 Tracings represent the means of the thrombotic profiles obtained from female (upper panel) and male (lower panel) mice (n = 7, ± SEM). Arrows indicate the time of appearance of the first thrombus. (B) Timing of cessation of tail bleeding in wt (n = 10), SLAM–/– (n = 20), and SAP–/– (n = 16) mice performed as previously described.1 No sex differences were observed in any group. Means depicted by (horizontal lines).
