Figure 3.
![Figure 3. Role of ceramide in TNFα-induced activation of JNK. (A) KG1 cells were prelabeled with [3H]-palmitic acid for 48 hours, washed, and treated with TNFα (20 ng/mL) at the indicated time. Intracellular levels of ceramide were analyzed as described in “Materials and methods.” Results are expressed as the mean values from 3 independent experiments ± SDs. *P < .05. (B) KG1 cells were preincubated or not for 1 hour with JNK inhibitor SP600125 (20 μM) then treated or not with C6-ceramide (10 μM) for 2 hours. Transcripts of hTERT were quantified using the LightCycler TeloTAGGGhTERT quantification kit as previously described. Results are expressed as mean ± SD from 4 independent experiments. ****P < .001; *P = .03.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/9/10.1182_blood-2005-04-1386/6/zh80210586240003.jpeg?Expires=1722710227&Signature=QOwNF2FxF6gPsLf~lVIcyRe3EnXMxytHUkGh5pwX8mChR3IaOGUqu1BQO2laKOLdWMqYGQ2Q1hRgJJXeFVfSRBu1XqBahbxlsxLNZnp-3AngjRGGqDNLDkeY4BdltdcXof0ESnWtOgV~3L1bGSy5duclXWEuh~jLyMik-M6-RnUuJj~I2RVqH1YgCWBpz3lhQaB6LIj~X69j3Sw~gdXPtXHA1JuVHj6Ftdmn5xRSf5TgHLGCZ~RI3aNitT6Ol0UPj3tefpRXpzHJ8J0ngRV3vbYkVJR5xR1Nx8dR35hZXBEqLhIORxiZgQNCMNEDCVC8jkdCRaasnTOqYHyABJm1EA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Role of ceramide in TNFα-induced activation of JNK. (A) KG1 cells were prelabeled with [3H]-palmitic acid for 48 hours, washed, and treated with TNFα (20 ng/mL) at the indicated time. Intracellular levels of ceramide were analyzed as described in “Materials and methods.” Results are expressed as the mean values from 3 independent experiments ± SDs. *P < .05. (B) KG1 cells were preincubated or not for 1 hour with JNK inhibitor SP600125 (20 μM) then treated or not with C6-ceramide (10 μM) for 2 hours. Transcripts of hTERT were quantified using the LightCycler TeloTAGGGhTERT quantification kit as previously described. Results are expressed as mean ± SD from 4 independent experiments. ****P < .001; *P = .03.
