Validation of predictive genes by quantitative real-time PCR. Nine healthy controls, 22 MDS, and 5 non-MDS anemias were studied. The horizontal bars represent median values. Significance values are shown for the comparison of normal (N) versus MDS, N versus non-MDS anemia (NMA), and MDS versus NMA. Genes tested were CD24 (A), VPREB1 (B), IL7R (C), POU2AF1 (D), PAX5 (E), LEF1 (F), MME (CD10) (G), IgHM (H), and IgHD (I).