FucTVII^{–/ –} α_E⁺ Tregs cannot enter the inflamed footpad and show no suppression of the Th1-mediated DTH reaction. (A) Expression of P-selectin–binding ligands on indicated CD4⁺ T-cell subsets from DO11.10 (WT) and DO11.10×FucTVII^–/–mice is shown. Representative histogram plots from 3 independently analyzed mice were selected. Numbers indicate frequency of P-selectin–binding cells. (B) In vitro preactivated Treg subsets from DO11.10 (WT) and DO11.10×FucTVII^–/– mice were radioactively labeled with 111In and injected intravenously into BALB/c mice, in which 24 hours before a DTH response had been induced, followed by the determination of radioactivity in the indicated organs after 24 hours using a γ counter. Percentage of total recovered radioactivity is shown (n = 12; mean ± SD; data pooled from 2 independent experiments; **P < .01). (C) In vitro generated Th1 cells (5 × 105) were injected intravenously together with 2.5 × 105 FACS-sorted and preactivated α_E⁺ Tregs from DO11.10 (WT) and DO11.10 × FucTVII^–/– mice. Twenty-four hours later the DTH response was induced. Shown is the progression of the inflammatory response monitored by the thickness of footpads injected with OVA/IFA (mean ± SD; n = 6). Two individual experiments are depicted. The α_E⁺ Tregs from FucTVII^–/– mice showed a significantly reduced suppressive capacity compared to α_E⁺ Tregs from WT mice (P < .01, repeated measure analyses).