Effects of conditioned medium derived from HCs grown in suspension on CFU-F and CFU-O development. BM-MNCs were plated directly in CFU-F and CFU-O assay conditions that used conditioned medium harvested from day 21 suspension cultures initiated with CD45⁺ cells that were supplemented with and without SCF plus IL-3. Statistical significance (P < .05) is denoted by an asterisk. Each bar represents the mean ± SD (n = 2 BM samples, performed in triplicate).