TCR-transduced patient T cells show WT1-specific killing activity and IFN-γ production.TCR-transduced T cells of a patient with CML (A) and AML (C) are able to kill T2 cells coated with pWT126 (pWT235 served as control) and the A2⁺ WT1⁺ leukemia line BV173. (B,D) T cells from the same CML and AML patients transduced with an irrelevant TCR (specific for Kb ⁺ pMDM100) failed to kill any of these targets. (E) TCR-transduced patient T cells show antigen-specific IFN-γ production upon stimulation with T2 cells coated with pWT126 peptide and the A2⁺ WT1⁺ leukemia line BV173. Peptide pWT235 served as control. Error bars indicate standard deviation of triplicates.