Specificity of TCR-transduced T cells. (A) Cytotoxicity of TCR-transduced T cells against T2 target cells pulsed with pWT126 and the control A2-binding peptide pWT235. (B) Peptide titration of purified TCR-transduced Vb2⁺CD8⁺ T cells compared with the parental CTL line 77 from which the TCR genes were isolated. (C) Cytotoxicity of TCR-transduced T cells against HLA-A2⁺ leukemia lines (697, BV173, and LAMA81). HLA-A2^– control Kyo-1 leukemia cells and T2 control targets. We have previously shown that all of these leukemia cell lines express WT1. (D) TCR-transduced T cells show antigen-specific cytokine production upon stimulation with T2 cells pulsed with pWT126 and pWT235 control peptide, or with A2⁺ leukemia cell lines and A2^– control cells. Error bars indicate standard deviation of triplicates.