Figure 1.
Figure 1. Colonies formed under various conditions. Top and bottom panels show the appearance of colonies under an inverted microscope (ITM; Olympus, Tokyo, Japan) equipped with a camera module (DP50; Olympus) (objective lens, SPlan 4PL; numerical aperture, 0.13; magnification, × 4) and their constituent cells stained with May-Grünwald-Giemsa solution (microscope, BX51, Olympus; camera module, XC-003, Sony, Tokyo, Japan; objective lens, UPlan F1, Olympus; numerical aperture, 1.3; magnification, × 100), respectively. (A) A spontaneous GM colony in the culture of JMML BM cells without hematopoietic factors. (B) A GM colony in the culture of normal BM cells with 10 ng/mL GM-CSF. (C) A spontaneous G colony in the culture of JMML BM cells with 10 μM ZOL.

Colonies formed under various conditions. Top and bottom panels show the appearance of colonies under an inverted microscope (ITM; Olympus, Tokyo, Japan) equipped with a camera module (DP50; Olympus) (objective lens, SPlan 4PL; numerical aperture, 0.13; magnification, × 4) and their constituent cells stained with May-Grünwald-Giemsa solution (microscope, BX51, Olympus; camera module, XC-003, Sony, Tokyo, Japan; objective lens, UPlan F1, Olympus; numerical aperture, 1.3; magnification, × 100), respectively. (A) A spontaneous GM colony in the culture of JMML BM cells without hematopoietic factors. (B) A GM colony in the culture of normal BM cells with 10 ng/mL GM-CSF. (C) A spontaneous G colony in the culture of JMML BM cells with 10 μM ZOL.

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