Effect of RGS16 knocking down on CFU-MK and platelet production. Cord-blood CD34⁺ cells were cultured in MK medium with TPO and SCF. Cells were transduced with pRRL-SCR or pRRL-SEQ24 and sorted using GFP, CD34, CD41 markers. (A) CFU-MK assay. pRRL-SCR or pRRL-SEQ24 CD34⁺/GFP⁺ sorted cells were grown in plasma clot in the presence of TPO and SCF. The histograms show the number of CFU-MKs obtained after 12 days of culture for 2000 cells plated per dish. (B) Proplatelet production. pRRL-SCR or pRRL-SEQ24 CD41⁺/GFP⁺ sorted cells were plated in the presence of TPO and SCF. The histograms represent the number of MKs and proplatelets found per well. Data represent the mean ± SD of 3 independent experiments.