Inhibition of RGS16 expression in primary MKs. Cord-blood CD34⁺ cells were cultured in MK medium with TPO and SCF. Cells were transduced with pRRL-SCR or pRRL-SEQ24 and sorted using the GFP and the CD41 marker. (A) Quantitative RT-PCR of CD41⁺/GFP⁺ and CD41⁺/GFP^– sorted cells. RGS16 expression was normalized with the β2m one. (B) SDF-1–induced migration of MK pRRL-SCR and MK pRRL-SEQ24. Migrated cells were marked with anti-CD41 and anti-CD42 antibodies for flow analysis. The data represent the percentage of CD41⁺/CD42⁺ migrated cells compared with CD41⁺/CD42⁺ total input cells. The graph shows the mean ± SD of 4 independent experiments performed in duplicate. *P < .01 (C) Cell adhesion. pRRL-SCR and pRRL-SEQ24 CD41⁺/GFP⁺ sorted cells were plated in 48-well plates coated with collagen I or fibronectin in the presence or not of SDF-1 (300 ng/mL). Data represent the mean ± SD of 3 independent experiments.