Inhibition of RGS16 expression. (A) Construction of an expression plasmid bearing shRNA and a lentiviral expression vector coding for shRGS16. (B) Test of different sequences of shRGS16 in 293 EBNA cells. 293 EBNA cells were cotransfected with pBS-H1 bearing different sequences of shRGS16 and MIGR as a marker of transfection efficiency. EBNA cells were sorted as shown on the GFP level. GFP⁺ cells were analyzed for RGS16 expression by quantitative RT-PCR using β2m as reference. The graph shows the mean ± SD of 4 independent experiments.