Overexpression of RGS16 and RGS18 in MO7e cell line. MO7e cells were infected with retroviruses containing RGS16 or RGS18. These and the control cells, which contain only the GFP, were sorted and analyzed. (A) Level of overexpression of RGS16 in MO7e RGS16 cells. Quantitative RT-PCR was performed, and RGS mRNA level was compared with the β2m mRNA level. This histogram represents the mRNA quantity of exogenous RGS in MO7e RGS16 and in MO7e RGS18. (B) Level of overexpression of RGS18 in MO7e RGS18 cell line. Quantitative RT-PCR was performed, and RGS mRNA level was compared with the β2m mRNA level. This histogram represents the mRNA quantity of RGS in MO7e RGS16 and MO7e RGS18 cells. (C) SDF-1–induced migration of MO7e GFP, MO7e RGS16, and MO7e RGS18 cells. Data represent the percentage of migrated cells compared with total input cells. The graph shows the mean ± SD of 4 independent experiments performed in duplicate. *P < .01. (D) Effect of RGS protein overexpression on the Erk and AKT activation pathway. MO7e GFP, MO7e RGS16, and MO7e RGS18 cell lines were starved of serum and GM-CSF for 12 hours and were stimulated or not with 300 ng/mL SDF-1 for the times indicated. (D) Erk and AKT activation was analyzed by Western blot analysis using an anti–phospho-Erk (P-Erk) antibody and an anti–phospho-AKT (P-AKT) antibody. Protein loading was assayed by labeling with an anti-Erk antibody (Total Erk) and an anti-AKT antibody (Total AKT).