Urine hepcidin profiles by SELDI-TOF-MS, and urine hepcidin levels in different iron metabolism disorders. (A) SELDI-TOF-MS spectra from urine samples of a septicemia patient (i), healthy subject (ii), and human synthetic hepcidin-25 (iii). Urine hepcidin-25 peaks from spectra shown in panel i and panel ii correlate with the reference human synthetic peptide (spectrum shown in panel iii). The annotated peak masses correspond with hepcidin-20, -22, and -25.²  (B) Urinary hepcidin excretion in patients with transfusion-induced iron overload (□), treated hereditary hemochromatosis (▴), iron deficiency anemia (×), endotoxemia after LPS injection (^*), and sepsis (○) compared with healthy subjects (♦). In each group the median is indicated.