Lactobacillus plantarum induces the translocation of MoDCs across the epithelial monolayer. MoDCs were seeded on the upper face of transwell filters facing the basolateral membrane of Caco-2 cells (see schematics on the left). Bacteria were seeded apically. Transwell filters were fixed 2 hours after addition of L plantarum (LP) or S typhimurium (SL-WT) or medium (CTRL) and were processed for laser confocal microscopy analysis. Green stains DCs (CD11c); red stains occludin of tight junctions. Right panels: Z sections of the filters, left panel: horizontal section (hs) of the apical face of the filter illustrated on the right showing DC dendrites in close contact with epithelial occludin. For each situation, 1 of 6 transwells is shown.