DAPI-banding analysis of MART-127-35–specific, hTERT-transduced CTL 4-TG. (A) DAPI-banded karyogram of a representative metaphase (n = 28) derived from CTL 4-TG at 2 months after hTERT transduction, indicating a normal 46, XX karyotype. (B) DAPI-banded karyogram of a metaphase derived from 4-TG at 10 months after hTERT transduction, showing a dicentric chromosome (arrow), consisting of a complete chromosome 4 fused to a centromere containing 5q, found in 1 cell of 24 cells analyzed.