American Society of Hematology

Figure 7.

$Figure 7. GzmB is only expressed in fractions of CD4+ and CD8+ PBMCs, but not in CD66b+CD4-CD8- PMNs of 4 patients and 3 healthy individuals. (A) PBMCs and PMNs were isolated from 4 patients (1-4) and 3 healthy individuals and analyzed (FACSCalibur) by staining with either mAb to CD4 and CD8 (PBMCs and PMNs), or mAb to CD66b (PMNs), and subsequently with αhugzmB mAb (1:50) as described in “Materials and methods.” Numbers in graphs indicate the percentages of gated cells. (B) Similarly prepared PBMCs and PMNs from another individual showing a comparable phenotype as the healthy individuals mentioned in panel A were centrifuged on microscope slides and stained with May-Grünwald/Giemsa as described in “Materials and methods.” Arrowheads indicate mononuclear cells; arrows, polymorphonuclear leukocytes.$

GzmB is only expressed in fractions of CD4⁺ and CD8⁺ PBMCs, but not in CD66b⁺CD4^-CD8^- PMNs of 4 patients and 3 healthy individuals. (A) PBMCs and PMNs were isolated from 4 patients (1-4) and 3 healthy individuals and analyzed (FACSCalibur) by staining with either mAb to CD4 and CD8 (PBMCs and PMNs), or mAb to CD66b (PMNs), and subsequently with αhugzmB mAb (1:50) as described in “Materials and methods.” Numbers in graphs indicate the percentages of gated cells. (B) Similarly prepared PBMCs and PMNs from another individual showing a comparable phenotype as the healthy individuals mentioned in panel A were centrifuged on microscope slides and stained with May-Grünwald/Giemsa as described in “Materials and methods.” Arrowheads indicate mononuclear cells; arrows, polymorphonuclear leukocytes.

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