Restoration of PTEN expression in Jurkat cells down-regulated IGF1-mediated chemotaxis. (A) The chemotactic responses of cultured PIJ-17 Jurkat cells (without dox) to different chemoattractants were tested. The concentrations used were determined previously as optimal for chemotaxis of different T lymphocyte subsets: RANTES (50 ng/mL), MDC (100 ng/mL), eotaxin3 (100 ng/mL), LARC (1000 ng/mL), IP-10 (100 ng/mL), I-309 (10 ng/mL), MIP-3β (100 ng/mL), IGF-1 (10 ng/mL), SDF-1α (100 ng/mL). The results were representative of 3 independent experiments. (B) PIJ-17 Jurkat cells were cultured with or without dox (1 μg/mL) for 48 hours and chemotaxis assays were performed as in panel A. The results were representative of 3 independent experiments. Data shown are means ± SD.