Elf1, Fli1, and Ets1 bind the conserved noncoding region. (A) Chromatin immunoprecipitation experiments were performed with anti-Elf1, -Fli1, -Ets1, and -Ets2 as well as control IgG antibodies. The DNA content of the immunoprecipitates was analyzed by real-time PCR for the presence of the conserved noncoding sequence in the proximal promoter region. The values plotted represent the level of enrichment with each antibody when compared with the levels obtained with the control IgG, plus or minus SD. (B) Real-time expression analysis of Elf1, Fli1, Ets1, and Ets2 transcripts in 416B and MS1 cell lines. The levels of expression of the different Ets factors are shown relative to Ets1, which was given an arbitrary value of 1 (note that Ets1 was expressed at significant levels in both cell lines) Error bars indicate standard deviation.