Effect of HHV-8 infection on HIV in primary endothelial cells. HUVEC cells were infected with HHV-8 or transfected with plasmid pCR-50sp, and 24 hours later were infected with HIV. Release of p24 antigen (A-C) and production of HIV infectious particles (D-F) were evaluated. Values represent the mean of duplicate samples of 2 separate experiments; (A,D) monotropic HIV_BaL strain (MOI, 1:100), (B,E) monotropic HIV_ADA strain (MOI, 1:100), (C,F) lymphotropic HIV_IIIB strain (MOI, 1:10). HIV production was measured by analyzing p24 release after coculturing infected HUVECs with C8166 target cells for 7 days. Error bars indicate SD. (G) Presence and transcription of HIV_IIIB in untreated (Control) and infected (HHV-8) HUVECs. Results are of PCR and rtPCR analyses by amplification of HIV gag gene.