Figure 1.
Function of mutant forms of SLC11A2. (A) HEK293T cells were transiently transfected with wild-type SLC11A2 (•), exon-12-deleted SLC11A2 (▪), E399D SLC11A2 (□) or vector alone (○). 55Fe uptake experiments were carried out twice, in duplicate or triplicate, as described previously.3 A representative experiment is shown. (B) Immunoblot analysis was performed with an anti-FLAG M2 monoclonal antibody (Sigma, Saint Louis, MO) recognizing an epitope tag.
