Survival of septic mice after siRNA treatment. (A) Survival of mice receiving saline, GFP siRNA, or caspase-8 siRNA 30 minutes after CLP (time of injection indicated by red arrow). Significant survival protection is seen at day 3. ^*P < .05 versus saline and GFP controls, chi-square test (n = 20/group). (B) Survival of mice receiving saline, GFP siRNA, or Fas siRNA 30 minutes after CLP. Significance in survival is also seen at day 3. ^*P < .05, chi-square test (n = 22 /group). (Ci) Percentage of survival of mice receiving a hydrodynamic injection (2 mL) of normal saline 12 hours after CLP, as compared with normal resuscitation, 0.8 mL lactated Ringer solution immediately following CLP. (Cii) The effect of Fas siRNA given hydrodynamically 12 hours after CLP (injection time indicated by arrow). A significant survival benefit is seen at day 5, ^*P < .05 versus saline and GFP controls, chi-square test (n = 15-17/group).