Attenuation of G_M1-ganglioside storage in the brain caused by exogenously delivered β-gal. (A) Thin-layer chromatography of hindbrain, midbrain, and forebrain (HMF), brain stem (BS), and cerebellum (CB) of Glb1^+/+, Glb1^-/-, and treated Glb1^-/- mice 3 months after BMT demonstrated amelioration of G_M1-ganglioside storage in the brain stem and cerebellum of the treated mice. The attenuation of G_M1-ganglioside accumulation was in agreement with the increment of β-gal activities in those areas (bottom row). G_M1-ganglioside was used as a standard. (B) Confocal microscopy with anti-G_M1 antibody confirmed less G_M1 accumulation in the cerebellum, brain stem, and hippocampus of treated mice (Glb1^-/- BMT^β-gal) than in Glb1^-/- mice that underwent mock transplantation (Glb1^-/- BMT^GFP). The arrows indicate cells in which G_M1-ganglioside accumulation was most arrested. Size bar corresponds to 50 μm. Image acquisition was performed as described for Figure 3.