The pHI-Db minigene vaccine induced suppression of angiogenesis determined by Matrigel assay. Quantification of vessel growth and staining of endothelium was determined by fluorometry and confocal microscopy, respectively, using FITC-labeled isolectin B4. (A) Representative Matrigel plugs were examined by confocal microscopy (original magnification × 200; 1.3 NA). The arrows indicate the borders of the Matrigel plug. (B) The average fluorescence of Matrigel plugs from each group of mice is depicted by the bar graph (n = 4; mean + SD). ^*P < .05 pCMV or pHI groups. The experiment was repeated once with similar results.