Figure 6.
![Figure 6. Colocalization of PGRP-S with lysozyme in the NETs. Neutrophils were activated with 100 nM PMA for 30 minutes and stained for DNA (A), PGRP-S (B), and lysozyme (C). For DNA detection, Hoechst 33342 (blue) was used. PGRP-S was detected with anti-human PGRP-S monoclonal antibody (mouse immunoglobulin G [IgG]) and Cy2-conjugated donkey anti-mouse IgG (green). Lysozyme was detected with anti-human lysozyme polyclonal antibody (sheep IgG) and Cy5-conjugated donkey anti-sheep IgG (red). The merged image (D) clearly shows colocalization of PGRP-S with lysozyme in the NETs.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/7/10.1182_blood-2005-02-0530/6/zh80190584830006.jpeg?Expires=1724275162&Signature=F9944pN5DOXkQ8~sIeybtkGW3vBvHs~4k5iQuwvjgq6swPzUHR6ffkM76XKeVeAX6NRRMbkfq3aRtsbMuvy7MCDwv5tFORZt68QjAvGB7sP05Mc5NZrbEHjdoRAzhQ83sxhkUVUx2SIYdL4t1kIZ5CMfz3tNvXGIJl04rdJxIO8Q~v6JM-Yu5f3kSd1WSOCuGydPd5cM7ZxGEqQSetVPMAKDCFZ9XcRy7xMkqjnOWms3VqFXmcITOxsAtIMhhc6RWI7L2F3xTN7fLPXdtxp3vezPn-IY-YZWNYB2DmBvvez2NhYXmY96wwbSh4VMfyeJ4t6rcm0LKVpiEe0kq3Glrw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Colocalization of PGRP-S with lysozyme in the NETs. Neutrophils were activated with 100 nM PMA for 30 minutes and stained for DNA (A), PGRP-S (B), and lysozyme (C). For DNA detection, Hoechst 33342 (blue) was used. PGRP-S was detected with anti-human PGRP-S monoclonal antibody (mouse immunoglobulin G [IgG]) and Cy2-conjugated donkey anti-mouse IgG (green). Lysozyme was detected with anti-human lysozyme polyclonal antibody (sheep IgG) and Cy5-conjugated donkey anti-sheep IgG (red). The merged image (D) clearly shows colocalization of PGRP-S with lysozyme in the NETs.
