Effect of the R59949 inhibitor on NIH-EGFR/ALK, 32D-NPM/ALK, and Karpas 299 cell growth. (A-B) NIH-EGFR/ALK cells were serum starved for 72 hours and then pretreated for 30 minutes with 1 μM R59949 (▪) or DMSO (▦) as control, before stimulation with the indicated dose of EGF (A) or 1% FBS (B) in the presence of 4 μCi (0.148 MBq) [methyl-³H] thymidine/well for 22 hours. Results are expressed as percent of control (radioactive incorporation obtained from medium + DMSO-treated cells). (C-D) 32D NPM/ALK and Karpas 299 cells were cultured for 48 hours in medium containing FBS 0.1% and BSA0.2 mg/mL in the presence of 10 μM R59949 (▪) or DMSO 0.02% (▦) as control. Cell proliferation was determined using the MTT proliferation assay. The values represent the means ± SD (n = 3). OD indicates optical density.