Figure 1.
![Figure 1. αDGK activity in NPM/ALK-positive cells. Cell lines were cultured for 30 minutes to 1 hour at 37°C in the absence (-) or presence (+) of either 10 μM R59949 or 1 μM PP2 and lysed thereafter. Total cell lysates (A) or anti-αDGK immunocomplexes (B, upper panel) were analyzed for in vitro DGK enzymatic activity in the presence of [γ-32P] ATP and diolein as exogen substrate; lipid phase was separated by TLC. An aliquot from each anti-αDGK immunoprecipitate was analyzed by SDS-PAGE to ensure that equal amounts of αDGK were present during in vitro kinase assay (B, bottom panel). PA indicates phosphatidic acid.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/6/10.1182_blood-2005-01-0316/6/zh80180583880001.jpeg?Expires=1724238812&Signature=syU8KQp~KtS7BjaN9heWHZWlZt2lJiMXCOAZ7ZcuWld0qB-6Uzv7yL2Ti5-zth3cqI4W98GmM7IKit3FVf4r3bnRJqvXnrRbJ0BcqewuI~Vy8RpTkE0GSyDRwK7fu0M3an01supdfDw9ILoTCKvIsgDoCE3HCbVys1H8nltVk5vZJ09GJiVQkM8nUPrRKCdT8QNS0K5GM7DlFAc4JG6keMc8XpojbdWhQkTblRgXbuFlUGPfas0iIRtfm5IopQjY2p9fA1bySbfvfcuncJQem5mXPqIE-nVSQf228QEZ3hqDxnTC-IXuXZovyLQN3uYwGw4Qme4Z9oKMkql6oZAYnw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
αDGK activity in NPM/ALK-positive cells. Cell lines were cultured for 30 minutes to 1 hour at 37°C in the absence (-) or presence (+) of either 10 μM R59949 or 1 μM PP2 and lysed thereafter. Total cell lysates (A) or anti-αDGK immunocomplexes (B, upper panel) were analyzed for in vitro DGK enzymatic activity in the presence of [γ-32P] ATP and diolein as exogen substrate; lipid phase was separated by TLC. An aliquot from each anti-αDGK immunoprecipitate was analyzed by SDS-PAGE to ensure that equal amounts of αDGK were present during in vitro kinase assay (B, bottom panel). PA indicates phosphatidic acid.
