Figure 2.
![Size distribution of HS. HSs were purified separately from each proteoglycan peak that was resolved by anion-exchange HPLC, and subjected to gel filtration chromatography on a CL-6B column, as described in “Materials and methods.” Three regions containing large-, intermediate-, and small-sized molecules were identified, based on the elution profile of normal intracellular HPLC peak no. 1 HS (Kav 0.1-0.4, 0.4-0.55, and 0.55-0.9, respectively [Kav = (elution volume - void volume)/(total volume - void volume)]). The proportion of HS present in each of these regions was calculated as a percentage of the total, for HS from intracellular peak no. 2 as well as HS from HPLC peaks no. 1 and no. 2 from the ECM.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/106/6/10.1182_blood-2005-02-0657/6/zh80180584120002.jpeg?Expires=1722025696&Signature=NtFfjtdCVn~bLgX71K0iaaASN~BxOK~sJoCxce~ZdUenw5h8Dnn8SlJA8bXoWiFxWV2plbJRwFJXs2ot4G8mEhFkKXkLsSwjwKfTMl5KLV9~MCk2HEdgmJJm2WXY-QxdKZ-b6Ect-XE16IdIYt5zn516~zjFrSMqK1QuraIezujW94QLSHYrMXM5CXd8e6yFftIFq~yaFL5qMyEvxcZdm6mD6lY9N3Hd9x1mYDhDnj84WdBDRh3gRC2slsvjR3gS5nIOf0rNAa1pJmWXvdLBYvC1-BsESTPDj2N9QrYq4zp8pCBoSlEUTRbJ-uQPAsWyPpGgTlI0qpLM6TRahKwN3g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Size distribution of HS. HSs were purified separately from each proteoglycan peak that was resolved by anion-exchange HPLC, and subjected to gel filtration chromatography on a CL-6B column, as described in “Materials and methods.” Three regions containing large-, intermediate-, and small-sized molecules were identified, based on the elution profile of normal intracellular HPLC peak no. 1 HS (Kav 0.1-0.4, 0.4-0.55, and 0.55-0.9, respectively [Kav = (elution volume - void volume)/(total volume - void volume)]). The proportion of HS present in each of these regions was calculated as a percentage of the total, for HS from intracellular peak no. 2 as well as HS from HPLC peaks no. 1 and no. 2 from the ECM.
