Immunofluorescence assay for SARS-CoV detection in human DCs. Mock-infected human DCs were included as a control (A). Positive immunofluorescence staining was detected in human immature and mature DCs at 12 hours (B) and 24 hours (C) after infection with SARS-CoV (MOI = 1). Confocal microscopy showed positive staining in the cytoplasm of DCs (D). Images are representative of immature and mature DCs from 11 independent adult or CB donors.