Glomerulonephritis in EAV is pauciimmune. Glomerular Ig deposition was assessed using direct immunofluorescence on 3-μm frozen sections. (A) Linear staining for IgG in a rat with experimental autoimmune glomerulonephritis, a model of antiglomerular basement membrane disease. This serves as a positive control; (B) scanty Ig deposits in a rat with EAV; (C) negative staining in a rat immunized with CFA alone. Images were captured using a confocal microscope, and bars represent 100 μm (× 20).