Autocrine-negative regulation of immature DC differentiation by prereceptor GC amplification. CD1a and CD14 expression following 7-day incubation of monocytes in the presence of IL-4/GM-CSF with vehicle or 10^-7 M cortisol or 10^-7 M cortisone ± GA. Top panel shows staining of input cells. Bottom panels show staining of cells on day 7 after culture. Figures represent percentage of cells in relevant quadrant. Similar results were obtained in 3 independent experiments.