Human monocyte-derived immature DCs express 11β-HSD1. (A) 11β-HSD1 and 11β-HSD2 mRNA expression (normalized to 18S rRNA) in monocytes and day 7 monocyte-derived immature DCs (imDC) (data shown as mean ± SEM; ^***P < .001 for 11β-HSD1 in imDCs versus monocytes, n = 10). (B) Confocal images showing staining for 11β-HSD1 (green) and the nucleus (4′, 6-diamidino-2-phenylindole, blue) in monocytes and imDCs (images representative of 3 independent experiments). (C) GRα and GRβ mRNA expression in monocytes and day-7 imDCs (data shown as mean ± SEM; n = 3).