![Figure 1. Effect of immune thrombocytopenic purpura (ITP) plasma on megakaryocyte production and maturation. / 1A. CD34+-rich cells were cultured in media containing marrow growth and development factor and either ITP or control plasma. After harvesting the cultures on day 10, the total number of megakaryocytes was determined [cell number (determined by direct counting of each culture) × % megakaryocytes (CD41+ cells by FACS)]. % Suppression = 100 – [mean total megakaryocytes in ITP cultures/mean total megakaryocytes in control cultures × 100]. The results of the 12 of 18 ITP plasmas tested, which showed significant suppression of megakaryocytopoiesis, are shown. / 1B. The histograms show the megakaryocyte ploidy distribution of CD34+ cells cultured for 10 days in the following plasmas (left to right): control, ITP-1 and ITP-2. Cells cultured in control or ITP-2 plasma (no evident suppression of in vitro megakaryocyte production) show 4 distinct peaks (2N, 4N, 8N, 16N) while cells cultured in ITP-1 plasma (suppressed in vitro megakaryocyte production) show primarily 2N cells. / Reprinted by permission from McMillan et al. Blood. 2004;103:1364–1369.](https://ash.silverchair-cdn.com/ash/content_public/journal/hematology/2004/1/10.1182_asheducation-2004.1.390/4/cines_fig1.jpeg?Expires=1722563550&Signature=NSjCI7TUkO2QaaOE5Op~qDmCb24eSoZ5chJRo4elwO9OJCTVsCu01uSztU4ULIevrqS~VItP1JjqpnHmsSqiw-CF1NXSTDEPZNv73~N-JzA5NInk-DNEo1YXhba8CsGlkuQC--5az2hdx2gBA~pRnI6DToQnRjdVhLRYK5Ku9vgwobKZTONzZVEaLXcDRfadABrnrqhWfkkm7aw3xnUMNELheAUufeNIytwyGVn1exJH-LbCBhTFYSRqnv-w4U1a4PDoH9tMhGzWwfR3KKc9Jm2K3gW4xiMhNMO5lvA6kqZZfDXbplU5zM4UWqeNgoDkDsEgHmVZNoSrvnjV4V2gqw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of immune thrombocytopenic purpura (ITP) plasma on megakaryocyte production and maturation.
1A. CD34+-rich cells were cultured in media containing marrow growth and development factor and either ITP or control plasma. After harvesting the cultures on day 10, the total number of megakaryocytes was determined [cell number (determined by direct counting of each culture) × % megakaryocytes (CD41+ cells by FACS)]. % Suppression = 100 – [mean total megakaryocytes in ITP cultures/mean total megakaryocytes in control cultures × 100]. The results of the 12 of 18 ITP plasmas tested, which showed significant suppression of megakaryocytopoiesis, are shown.
1B. The histograms show the megakaryocyte ploidy distribution of CD34+ cells cultured for 10 days in the following plasmas (left to right): control, ITP-1 and ITP-2. Cells cultured in control or ITP-2 plasma (no evident suppression of in vitro megakaryocyte production) show 4 distinct peaks (2N, 4N, 8N, 16N) while cells cultured in ITP-1 plasma (suppressed in vitro megakaryocyte production) show primarily 2N cells.
Reprinted by permission from McMillan et al. Blood. 2004;103:1364–1369.
