Platelet CypD mediates the recruitment of neutrophils to the brain following cerebral ischemia-reperfusion injury. Male and female CypD^plt−/− mice or littermate controls (CypD^plt+/+) were subjected to 1 hour of cerebral ischemia followed by 23 hours of reperfusion (tMCAO). (A) Representative dot plots displaying gating strategy for the flow cytometric quantification of neutrophils recruited to the brain. Neutrophils were identified as single cells that were CD45^high, CD11b⁺, and Ly6G⁺. (B) Quantification of the number of neutrophils in the ischemic hemisphere analyzed by flow cytometry 24 hours after stroke. (C) Representative images of neutrophil (Ly6G, magenta) staining in ischemic brain tissues 24 hours after stroke. Neutrophils are denoted by white arrows. Blue staining shows nuclei (DAPI). Boxes (in left panels) are shown enlarged (right panels). (D) Quantification of the number of neutrophils in ischemic brain tissues analyzed by immunohistochemistry 24 hours after stroke. Four sections throughout the brain were analyzed in each mouse.